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. 2023 May 30;14:1188215. doi: 10.3389/fphar.2023.1188215

TABLE 3.

The clinical studies of natural compounds against OA development by mediating the NRF2/ARE signaling pathway.

Compounds Models/Doses Biological actions Conclusions Ref
Genistein IL-1β-treated human chondrocytes/10 μM NRF2↑, HO-1↑, COX-2↓, iNOS↓, MMP-1↓, MMP-3↓, MMP-13↓, collagen II↑, aggrecan↑ Inhibits inflammation and ECM degradation by activating the NRF2/ARE signaling Liu et al. (2019)
Myricetin IL-1β-treated human chondrocytes/5, 10, and 15 μM NRF2↑, HO-1↑, p65↓, IL-6↓, TNFα↓, COX-2↓, PGE2↓, iNOS↓, NO↓, MMP-13↓, ADAMTS5↓, collagen II↑, aggrecan↑ Inhibits inflammation and ECM degradation by activating the NRF2/ARE signaling Pan et al. (2019)
Morin IL-1β-treated human OA chondrocytes/2.5, 5, and 10 μM PGE2↓, NO↓, p65↓, NRF2↑, HO-1↑, MMP-1↓, MMP-3↓, MMP-13↓ Inhibits inflammation and ECM degradation by activating the NRF2/ARE signaling Qu et al. (2018)
Wogonin IL-1β-treated human OA chondrocytes/10, 25, and 50 μM IL-6↓, TNFα↓, COX-2↓, PGE2↓, iNOS↓, NO↓, MMP-3↓, MMP-9↓, MMP-13↓, ADAMTS5↓, collagen II↑, ACAN↑, NRF2↑, HO-1↑, NQO-1↑, p-ERK1/2↑ Inhibits inflammation and ECM degradation by activating the NRF2/ARE signaling Khan et al. (2017)
Polydatin IL-1β-treated human chondrocytes/25, 50, and 100 μg/mL NRF2↑, HO-1↑, IL-6↓, TNFα↓, COX-2↓, PGE2↓, iNOS↓, NO↓, MMP-13↓ Inhibits inflammation and ECM degradation by activating the NRF2/ARE signaling Tang et al. (2018)
Curcumin IL-1β-treated human chondrocytes/20 and 40 μM IL-6↓, TNFα↓, COX-2↓, iNOS↓, MMP-1↓, MMP-3↓, MMP-9↓, MMP-13↓, ADAMTS4↓, ADAMTS5↓, COL2A1↑, ACAN↑, NRF2↑, HO-1↑, NQO-1↑, GCLC↑, SOD-2↑ Inhibits inflammation and ECM degradation by activating the NRF2/ARE signaling Jiang et al. (2020a)
Piceatannol IL-1β-treated human chondrocytes/1, 5, and 10 μM IL-6↓, TNFα↓, COX-2↓, PGE2↓, iNOS↓, NO↓, MMP-13↓, ADAMTS5↓, collagen II↑, aggrecan↑, p65↓, NRF2↑, HO-1 Inhibits inflammation and ECM degradation by activating the NRF2/ARE signaling Tang et al. (2017)
ICA IL-1β-treated HC-A/10−9M ROS↓, GPX↑, SOD↑, MMP-3↓, MMP-9↓, MMP-13↓, ADAMTS4↓, GAG↑, NRF2↑, NQO-1↑ Inhibits oxidative stress and ECM degradation by activating the NRF2/ARE signaling Zuo et al. (2019)
CA TNFα-treated C28/I2 cells/5, 10, and 20 μM IL-1β↓, IL-6↓, IL-12↓, COX-2↓, PGE2↓, iNOS↓, NO↓, MMP-3↓, MMP-9↓, MMP-13↓, ADAMTS5↓, collagen II↑, aggrecan↑, ROS↓, p-PI3K↓, p-AKT↓, p65↓ Inhibits inflammation, oxidative stress, and ECM degradation by activating the NRF2/ARE signaling Qu et al. (2022)
Hederagenin IL-1β-treated C28/I2 cells/5, 10, and 20 μM IL-6↓, TNFα↓, COX-2↓, PGE2↓, iNOS↓, NO↓, ROS↓, MMP-1↓, MMP-3↓, MMP-9↓, MMP-13↓, ADAMTS5↓, collagen II↑, aggrecan↑, Bax↓, Bcl-2↑, cleaved caspase-3↓, p-JAK2↓, p-STAT3↓, MAPK↓, NRF2↑ Inhibits inflammation, oxidative stress, chondrocyte apoptosis, and ECM degradation by activating the NRF2/ARE signaling Shen et al. (2022)
SFN H2O2-treated human OA chondrocytes/7 μM NRF2↑, NOX4↓, COL2↑, ACAN↑, SOX9↑, IL-6↓, TNFα↓, MMP-13↓, ADAMTS5↓ Inhibits inflammation, oxidative stress, and ECM degradation by activating the NRF2/ARE signaling Yang et al. (2020a)