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. 2023 Jun 14;11(6):e898. doi: 10.1002/iid3.898

Figure 6.

Figure 6

Interleukin‐38 (IL‐38) inhibited macrophage inflammation in the presence of troponin I (TnI). Macrophages were incubated with 10 ng/mL lipopolysaccharide (LPS) in the presence/absence of 1 μg/mL TnI and/or 50 ng/mL IL‐38 for 24 h. The expression levels of IL‐6 (A), tumor necrosis factor‐α (TNF‐α) (B), IL‐1β (C), and IL‐10 (D) were measured by enzyme‐linked immunosorbent assay (ELISA) in cultured supernatants of different treatment groups. (E) Protein was extracted from cultured cardiomyocytes. Representative images of a western blot for expression levels of p65NF‐κB and NOD‐like receptor pyrin domain‐related protein 3 (NLRP3) (n = 6). *p < .05 vs. sham (LPS+TnI−IL‐38−), **p < .01 vs. sham (LPS+TnI−IL‐38−); # p < .05 vs. (LPS+TnI+IL‐38−), ## p < .01 vs. (LPS+TnI+IL‐38−).