Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Principle of telomere PCR. Genomic DNA is isolated, denatured and tailed with dCTP in the presence of terminal deoxynucleotidyl transferase. The G-rich telomere strand is specifically amplified with a primer complementary to the dC tail and a primer specific for a sequence distal of the subtelomeric ADE2 gene. The PCR products are analysed for their length on agarose gels, then cloned and sequenced.
