Figure 2.

Comparison of telomere length analysis by Southern blotting and telomere PCR. (A) Southern blot of genomic DNA digested with XhoI and electrophoresed on a 0.7% agarose gel. The blot was hybridised with a Y′-element probe that corresponds to a subtelomeric repeat present at most yeast telomeres. Lanes 1 and 2 are independent DNA preparations from the same strain. (B) Telomere PCR of the same DNA samples as in (A), electrophoresed on 4% agarose gels. DNA from strain GA245 (no telomeric ADE2 gene) does not give a PCR product (lane 7). (C) Control reaction for the integrity of the genomic DNA. A primer pair amplifying the 3′ end of the internal ade2 gene yields a product of constant size in all yeast strains used in (B).