Table 1.
Comparison of PacBio and ONT sequencing technologies
| Sequencing technology | Platform | Supported flow cell | Data production | Read length (kbp) | Mean read accuracy (%) | Throughput per flow cell (Gbp) | Estimated Cost per Gbp (US$)a | Applications |
|---|---|---|---|---|---|---|---|---|
| Pacific Biosciences (PacBio) | Sequel II/IIec | SMRT Cell 8M | HiFi | 15-25 (up to 40) | >99 | 30-42 | 31-43 | WGS, Gene panels, cDNA sequencing, Methylation analysis, Metagenomics and microbiome analyses |
| Revioc | SMRT Cell 25M | >99 | Up to 90-126b | 8-11b | ||||
| Oxford Nanopore Technologies (ONT) | Flongle | R9.4.1 | Simplex, duplex | 1-100 (up to > 2000) | >95 | 1-2 | 118d | Amplicon or plasmid sequencing |
| MinION | R9.4.1, R10.4.1 | >95 | 15-25 | 29–51e | Amplicon sequencing, Sequencing of small genomes, Adaptive sampling, gene panel, cDNA and direct RNA sequencing, Metagenomic and microbiome analysis | |||
| GridION | R9.4.1, R10.4.1 | >95 | 15-25f | 29–51e | ||||
| PromethION | R9.4.1, R10.4.1 | >95 | 100-200 | 6–12g | WGS, cDNA and direct RNA sequencing, Adaptive sampling |
aPricing includes exclusively SMRTbell prep and sequencing reagents run on proprietary instruments.
bProjected estimate: Revio was launched in Q1 2023.
cThe Sequel IIe and Revio can process the raw sequencing data and generate HiFi reads on the instrument.
dAssumes output of 1.5 Gbp per flow cell using single library.
eAssumes output of 20 Gbp per flow cell using single library.
fGridION allows simultaneous sequencing on 5 flow cells.
gAssumes output of 150 Gbp per flow cell with three libraries.