TABLE 9.

Summary of MultiFlow DNA Damage Assay results

		Machine Learning (ML) Ensemble						Global Evaluation Factor (GEF) Rubric
		Aneugen Calls			Clastogen Calls
Chemical	+/−S9	RFa	ANNb	LRc	RF	ANN	LR	Overall ML Genotoxicity Calls (MoAd)	Aneugen Calls	Clastogen Calls	Overall GEF Calls (MoA)	Overall ML + GEF Calls (MoA)
Glyphosate	−S9	-	-	-	-	-	-	Non-genotoxic	-	-	Non-genotoxic	Non-genotoxic
	+S9	-	-	-	-	-	-	Non-genotoxic	-	-	Non-genotoxic	Non-genotoxic
Glyphosate IPA	−S9	-	-	-	-	-	-	Non-genotoxic	-	-	Non-genotoxic	Non-genotoxic
	+S9	-	-	-	-	-	-	Non-genotoxic	-	-	Non-genotoxic	Non-genotoxic
AMPA	−S9	-	-	-	-	-	-	Non-genotoxic	-	-	Non-genotoxic	Non-genotoxic
	+S9	-	-	-	-	-	-	Non-genotoxic	-	-	Non-genotoxic	Non-genotoxic
Diquat dibromide	−S9	-	-	-	+	+	+	Clastogen	-	-	Non-genotoxic	Clastogen
	+S9	-	-	-	+	+	+	Clastogen	-	+	Clastogen	Clastogen
Metolachlor	−S9	-	-	-	+	+	+	Clastogen	-	+	Clastogen	Clastogen
	+S9	-	-	-	-	-	-	Non-genotoxic	-	-	Non-genotoxic	Non-genotoxic
Mesotrione	−S9	-	-	-	-	-	-	Non-genotoxic	-	-	Non-genotoxic	Non-genotoxic
	+S9	-	-	-	-	-	-	Non-genotoxic	-	-	Non-genotoxic	Non-genotoxic
GBF e
Roundup Custom	−S9	-	-	-	+	+	+	Clastogen	-	-	Non-genotoxic	Clastogen
	+S9	-	-	-	-	+	-	Non-genotoxic	-	+	Equivocalf	Non-genotoxic
Halex GT	−S9	-	-	-	+	+	+	Clastogen	-	+	Equivocalf	Clastogen
	+S9	-	-	-	-	+	-	Non-genotoxic	-	-	Non-genotoxic	Non-genotoxic

^aRF = random forest.

^bANN = artificial neural networks.

^cLR = linear regression.

^dMode of action (MoA)

^eGlyphosate-based formulations (GBFs) other than Roundup Custom and Halex GT were identified as non-genotoxic by the ML ensemble and the global evaluation factor (GEF) rubric.

^fAlthough the GEF clastogen call is +, the positive result was not repeated and so the call is reported as “equivocal.”