Table 1.
Genotypic effects on meiotic metaphase I chromosomes of Ttzip4 single, double and triple mutants compared with those of Kr3161 TtZIP4-A1B1B2 control plants.
| Genotype | No. of cells scored | Univalents Mean ± SE (Range) |
Rod bivalents Mean ± SE (Range) |
Ring bivalents Mean ± SE (Range) |
Trivalents Mean ± SE (Range) |
Tetravalents Mean ± SE (Range) |
Single chiasmata Mean ± SE (Range) |
Double chiasmata Mean ± SE (Range) |
|---|---|---|---|---|---|---|---|---|
| ZIP4-A1B1B2 | 168 | 0.31 ± 0.06e
(0-2) |
1.74 ± 0.10d
(0-6) |
12.10 ± 0.10a
(8-14) |
0.00 ± 0.00a
(-) |
0.00 ± 0.00a
(-) |
25.94 ± 0.12a
(21-28) |
28.07 ± 0.12a
(23-31) |
| zip4-A1 | 127 | 0.57 ± 0.09de
(0-6) |
1.76 ± 0.11d
(0-6) |
11.95 ± 0.11a
(7-14) |
0.00 ± 0.00a
(-) |
0.00 ± 0.00a
(-) |
25.70 ± 0.14a
(20-28) |
27.42 ± 0.14a
(22-30) |
| zip4-B1 | 128 | 1.46 ± 0.14bc
(0-9) |
3.16 ± 0.13bc
(0-9) |
10.13 ± 0.15bc
(5-14) |
0.00 ± 0.00a
(-) |
0.00 ± 0.00a
(-) |
23.43 ± 0.20bc
(17-28) |
25.30 ± 0.17b
(20-29) |
|
zip4-B2
(ph1c) |
144 | 1.96 ± 0.12bc
(0-6) |
3.89 ± 0.13ab
(1-9) |
9.13 ± 0.14cd
(5-13) |
0.00 ± 0.00a
(-) |
0.00 ± 0.00a
(-) |
22.17 ± 0.17cd
(18-27) |
25.04 ± 0.20b
(20-31) |
| zip4-B2 CRISPR | 157 | 2.07 ± 0.13bc
(0-8) |
4.17 ± 0.13a
(0-9) |
8.76 ± 0.14d
(4-13) |
0.02 ± 0.01 a
(0-1) |
0.01 ± 0.01 a
(0-1) |
21.75 ± 0.18d
(15-26) |
22.68 ± 0.19c
(16-27) |
| zip4-A1B1 | 164 | 17.77 ± 0.33a
(8-26) |
4.48 ± 0.15a
(0-8) |
0.63 ± 0.06e
(0-4) |
0.00 ± 0.00a
(-) |
0.00 ± 0.00a
(-) |
5.75 ± 0.20e
(1-12) |
6.64 ± 0.24d
(0-14) |
| zip4-A1B2 | 108 | 1.22 ± 0.12cd
(0-4) |
2.83 ± 0.15c
(0-7) |
10.56 ± 0.17b
(6-14) |
0.00 ± 0.00a
(-) |
0.00 ± 0.00a
(-) |
23.94 ± 0.20b
(19-28) |
25.09 ± 0.22b
(19-29) |
| zip4-B1B2 | 156 | 2.28 ± 0.14b
(0-8) |
3.96 ± 0.12a
(1-8) |
8.88 ± 0.13d
(5-13) |
0.01 ± 0.01a
(0-1) |
0.01 ± 0.01a
(0-1) |
21.75 ± 0.17d
(17-27) |
23.83 ± 0.30c
(17-40) |
| zip4-A1B1B2 | 128 | 25.88 ± 0.20a
(18-28) |
1.04 ± 0.10d
(0-5) |
0.02 ± 0.01e
(0-1) |
0.00 ± 0.00a
(-) |
0.00 ± 0.00a
(-) |
1.08 ± 0.10e
(0-5) |
1.20 ± 0.13d
(0-8) |
| p-value | < 0.0001 | < 0.0001 | < 0.0001 | 0.0361 | 0.6277 | < 0.0001 | < 0.0001 |
The mean numbers of univalents, rod and ring bivalents, trivalents and tetravalents were scored along with chiasma frequency scored as single and double chiasmata. Standard error (SE) values are shown. The range is given in brackets. P-values < 0.05 indicate significant differences. Superscript letters a-e indicate where the significant differences lie. For scores with the same letter, the difference between the means is not statistically significant. If the scores have different letters, they are significantly different. Note particularly the high numbers of univalents in the Ttzip4-A1B1 double mutants and almost total univalence in the Ttzip4-A1B1B2 triple mutants, and the corresponding low levels of chiasma frequency.