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. Author manuscript; available in PMC: 2023 Jun 14.
Published in final edited form as: Immunity. 2022 Dec 28;56(1):107–124.e5. doi: 10.1016/j.immuni.2022.12.002

Figure 5. Co-stimulation from antigen-presenting cells promotes tumor-specific CD8+ T cell differentiation.

Figure 5.

(A) tSNE clustering of antigen-presenting cells (APCs) sorted from naive LN, TDLN, and tumors of 5-week TRAMPC1-GP-bearing mice.

(B) tSNE clustering split by tissue origin, with proportions of each cluster from each tissue represented in a bar graph; each APC population is significantly different between TDLN and tumor.

(C) Flow cytometry analysis of DC subsets from TDLN and tumor from TRAMPC1-GP-bearing mice.

(D) Flow cytometry analysis of co-stimulatory molecules in CD11b+ DCs in naive LNs, TDLNs, and tumors from TRAMPC1-GP-bearing mice.

(E) Experimental setup. P14s were transferred into 4-week TRAMPC1-GP tumor-bearing mice, and 5 days post transfer, they were treated with CpG and/or CD80/CD86 blocking antibodies for 10 days.

(F and G) Phenotype of transferred PD1+ CD44+ P14s 15 days post transfer in the TDLNs and tumors of all treatment groups (F) and summaries of P14 phenotype (G). Median and 95% confidence intervals (CIs) are shown. *p < 0.05 determined by Mann-Whitney test.