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. Author manuscript; available in PMC: 2023 Jun 14.
Published in final edited form as: Immunity. 2022 Dec 28;56(1):107–124.e5. doi: 10.1016/j.immuni.2022.12.002

Figure 7. Co-stimulation is necessary for the differentiation of human tumor-stem-like CD8+ T cells.

Figure 7.

(A) Experimental layout to test the requirement of co-stimulation to differentiate sorted tumor-stem-like CD8+ T cells.

(B) Summary plot shows the proportion of divided CD8+ T cells in each in vitro condition, based on CTV dilution.

(C) Representative plots showing CTV dilution and phenotype after 5 days of culture in each condition.

(D) Representative plots showing CTV dilution and expression of GZMB of control in vitro stimulations or with added IL-12.

(E) Histograms showing expression of TIM3, CD39, and Tbet for all conditions. Medians and 95% CIs are represented. *p < 0.05 determined by Mann-Whitney test. *p < 0.05 determined by Wilcoxon test when sufficient paired samples were analyzed, shown as connected by a line.