|
| Antibodies |
|
| anti-mouse CD11b (Fitc) |
Biolegend |
clone: M1/70, RRID: AB_312789 |
| anti-mouse MHCII (I-A/I-E) (A700) |
Biolegend |
clone: M5/114.15.2, RRID: AB_493727 |
| anti-mouse CD86 (BV421) |
Biolegend |
clone: GL-1, RRID: AB_10898329 |
| anti-mouse CD80 (BV605) |
Biolegend |
clone: 16-10A1, RRID: AB_11126141 |
| anti-mouse CD19 (BV650) |
Biolegend |
clone: 6D5, RRID: AB_11204087 |
| anti-mouse CD3 (BV650) |
Biolegend |
clone: 17A2, RRID: AB_11204249 |
| anti-mouse CD11c (BV711) |
Biolegend |
clone: N418, RRID: AB_2563905 |
| anti-mouse CD8a (Percp-Cy5.5, Pe-Cy7) |
Biolegend |
clone: 53–6.7, RRID: AB_2075238 |
| anti-mouse IRF4 (FITC, A647) |
Biolegend |
clone: IRF4.3E4, RRID: AB_2563266 |
| anti-mouse TCF1 (PE) |
BD |
clone: S33–966, RRID: AB_2687845 |
| anti-mouse CD127 (PE-Dazzle) |
Biolegend |
clone: A7R34, RRID: AB_2564216 |
| anti-mouse Tim3 (Pe-cy7, BV421) |
Biolegend |
clone: RMT3–23, RRID: AB_2571933 |
| anti-mouse Blimp1 (APC) |
Biolegend |
clone: 5E7, RRID: AB_2565618 |
| anti-mouse CD44 (A700, BV510) |
Biolegend |
clone: IM7, RRID: AB_2561391 |
| anti-mouse CD62L (BV605) |
Biolegend |
clone: MEL-14, RRID: AB_11125577 |
| anti-mouse CD45.1 (BV711) |
Biolegend |
clone: A20, RRID: AB_2562605 |
| anti-mouse PD1 (BV786) |
Biolegend |
clone: 29E.1A12, RRID: AB_2563680 |
| anti-mouse Ki67 (FITC, BV650) |
BD |
clone: B56, RRID: AB_2688008 |
| anti-mouse/human Gzmb (A700, BV421) |
BD |
clone: GB11, RRID: AB_1645453 |
| anti-mouse IFNy (APC) |
Biolegend |
clone: XMG1.2, RRID: AB_315403 |
| anti-mouse TNFa (FITC) |
Biolegend |
clone: MP6-XT22, RRID: AB_315425 |
| anti-mouse IL2 (PE) |
Biolegend |
clone: JES6-5H4, RRID: AB_315302 |
| Fixable Live Dead |
Thermo-Fisher |
Near IR, Aqua |
| anti-mouse Fc block |
Biolegend |
clone: 93, RRID: AB_1574975 |
| anti-human CD141 (FITC) |
Miltenyi Biotec |
clone: REA674, RRID: AB_2751167 |
| anti-human HLA-DR (PE, APC-Cy7) |
Biolegend |
clone: L243, RRID: AB_493586 |
| anti-human CD1c (BUV395, APC) |
Biolegend/BD |
clone: F10/21A3, RRID: AB_2741017 |
| anti-human CD11b (BUV737, BV605) |
Biolegend/BD |
clone: ICRF44, RRID: AB_2562020 |
| anti-human CD14 (BUV661) |
BD |
clone: M5E2, RRID: AB_2871011 |
| anti-human CD86 (BV786) |
Biolegend |
clone: IT2.2, RRID: AB_2616793 |
| anti-human CD11c (BV711) |
Biolegend |
clone: 3.9, RRID: AB_11219609 |
| anti-human CD40 (A700) |
Biolegend |
clone: 5C3, RRID: AB_2563921 |
| anti-human CD16 (Pe-Dazzle, BUV661) |
Biolegend/BD |
clone: 3G8, RRID: AB_2563638 |
| anti-human CD135 (PE) |
BD |
clone: 4G8, RRID: AB_397175 |
| anti-human PDL1 (BV605) |
Biolegend |
clone: 29E2A3, RRID: AB_2565926 |
| anti-human Tim3 (PE) |
R&D |
clone: FAB2365P, RRID: AB_2232901 |
| anti-human CD45 (APC) |
Biolegend |
clone: 2D1, RRID: AB_2566372 |
| anti-human CD3 (BUV395, BV510) |
Biolegend/BD |
clone: UCHT1, RRID: AB_2563468 |
| anti-human CD28 (Pe-Cy7) |
Thermofisher |
clone: CD28.2, RRID: AB_1944363 |
| anti-human PD1 (BUV737, BV786) |
Biolegend/BD |
clone: EH12.1, RRID: AB_2870118 |
| anti-human CD39 (BV421, BV786) |
Biolegend |
clone: A1, RRID: AB_2564575 |
| anti-human TCF1 (A488) |
Cell Signaling |
clone: C63D3, RRID: AB_2797627 |
| anti-human Ki67 (A647, FITC) |
BD |
clone: B56, RRID: AB_647087 |
| anti-human CD69 (Pe-Dazzle) |
Biolegend |
clone: FN50, RRID: AB_2564276 |
| anti-human CD127 (BV711) |
Biolegend |
clone: A019D5, RRID: AB_2562908 |
| anti-human CD45RA (APC, BV510) |
Biolegend |
clone: HI100, RRID: AB_314415 |
| anti-human CCR7 (APC, BV421) |
Biolegend |
clone: G043H7, RRID: AB_10915474 |
| anti-human CD25 (A647) |
Biolegend |
clone: M-A251, RRID: AB_2904482 |
| anti-human CD8 (Percp-Cy5.5, BUV395) |
Biolegend/BD |
clone: RPA-T8, RRID: AB_2874820 |
| human FcR Blocking Reagent |
Miltenyi Biotec |
clone: 130-059-901, RRID: AB_2892112 |
|
| Biological samples |
|
| Human Tumor Samples |
Emory University |
N/A |
| Mouse Tissue |
Emory University |
N/A |
|
| Chemicals, peptides, and recombinant proteins |
|
| Collagenase D |
Sigma |
11088882001 |
| GP33 peptide |
Genscript |
N/A |
|
| Critical commercial assays |
|
| eBiosciences Foxp3/Transcription Factor Staining Kit |
Thermo Fisher |
00–5523-00 |
| EasySep CD8 Mouse Negative Selection Kit |
Stem Cell |
19853 |
| IFNy Human ELISA kit |
Thermo Fisher |
KHC4021 |
|
| Deposited data |
|
| Human and mouse genomics data |
This Paper |
GEO: GSE216731
|
|
| Experimental models: Cell lines |
|
| TRAMPC1-LCMV-GP |
Emory University |
N/A |
| B16F10-LCMV-GP |
Emory University |
N/A |
| TRAMPC1 |
Emory University |
N/A |
| RENCA-HA |
Emory University |
N/A |
|
| Experimental models: Organisms/strains |
|
| Mouse:C57BL/6J |
The Jackson Laboratory |
000664 |
| Mouse:P14(B6;D2-Tg(TcrLCMV)327Sdz/JDvsJ) |
The Jackson Laboratory |
004694 |
|
| Software and algorithms |
|
| R: The Project for Statistical Computing |
N/A |
https://www.r-project.org/
|
| Seurat |
Satija et al.66
|
https://satijalab.org/seurat/
|
| DeSeq2 |
Love et al.67
|
https://bioconductor.org/packages/release/bioc/html/DESeq2.html
|
| Graphpad Prism |
GraphPad Prism |
N/A |
