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. 2023 May 31;11:1189010. doi: 10.3389/fbioe.2023.1189010

FIGURE 3.

FIGURE 3

FISH analysis of wound tissue from different groups of mice (Scale bar = 50 µm). The wound tissue of S. aureus was labeled by a specific ribosomal RNA (rRNA) FISH probe (Red). The nuclear region of the bacteria was stained (blue) after DAPI (4′,6-diamidino-2-phenylindole) counterstaining (A). Comparison of the number of viable bacteria in the wound tissues after treatment with the four different gels by dilution coating plate method (diluted 103 times, n = 3/group) (B). Quantitative analysis of IL-1β, IL-6, and TNF-α levels in wound tissue on day 5 after treatment (***p < 0.01, *p < 0.05 when compared with the model group, # p < 0.05 when compared with the BH-Lip gel group, n = 6/group) (C,D,E). Quantitative analysis of IL-1β, IL-6, and TNF-α levels in wound tissue on day 14 after treatment (***p, **p < 0.01, *p < 0.05 when compared with the model group, # p < 0.05 when compared with the BH-Lip gel group, n = 6/group) (F,G,H).