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. Author manuscript; available in PMC: 2023 Oct 12.
Published in final edited form as: Cell Host Microbe. 2022 Sep 20;30(10):1370–1381.e5. doi: 10.1016/j.chom.2022.08.018

Figure 2. The pro-inflammatory intestinal microbiome of Sp140−/− mice is transferable and exacerbates colitis.

Figure 2.

(A) Daily body weight of separately housed wildtype (WT) and Sp140−/− mice after 2% dextran sulfate sodium (DSS) administration (n=6). (B) Daily body weight of cohoused WT and Sp140−/− mice after 2% DSS administration (n=6). (C) Day 12 colon lengths of separated or cohoused WT and Sp140−/− mice after 2% DSS administration (n=6). (D) Representative hematoxylin and eosin-stained sections of day 12 distal colon tissue from separated or cohoused WT and Sp140−/− mice after 2% DSS administration. (E) Breeding scheme for F2 littermates used to normalize microbiota between WT and Sp140−/− mice. (F) Daily body weight of WT and Sp140−/− F2 littermates after 2% DSS administration (n=5). (G) IL-6 production, as determined by ELISA, of bone marrow-derived macrophages (BMDMs) supernatants after 16 hours stimulation with LPS (1mg/mL) or stool homogenates (1mg/mL) from WT (n=5), Sp140−/− (n=6), or broad-spectrum antibiotic treated WT mice (n=3); representative of 3 experiments. Error bars represent means ± SEM. n.s., not significant, *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001; two-way ANOVA for (A) and (B), one-way ANOVA for (C) and (E).