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. 2000 Jul 15;28(14):2702–2708. doi: 10.1093/nar/28.14.2702

Table 3. Fluorescent properties of selected 2AP containing oligodeoxynucleotides in the presence and absence of Mg2+.

  F/F0a F/F0a
X-Y (0.15 M Na+) (0.15 M Na+ + 15 mM Mg2+)
2AP-1 3.4 6.0
2AP-2 2.9 6.5
2AP-3 2.9 7.0
2AP-4 2.4 5.4
2AP-T 1 1
2AP-(-)b 12.6 15.5
2APc 6.6 7.1

aThe ratio of fluorescence intensities at 370 nm for the 2AP-Y duplexes (F) and the 2AP-T duplex(F0): Buffer cond. c(duplex) = 2 µM, in 10 mM NaH2PO4, pH 7.0.

bDuplex with 2AP residue in a single nucleotide bulge.

c2AP containing single strand.