Table 3. Fluorescent properties of selected 2AP containing oligodeoxynucleotides in the presence and absence of Mg2+.
F/F0a | F/F0a | |
---|---|---|
X-Y | (0.15 M Na+) | (0.15 M Na+ + 15 mM Mg2+) |
2AP-1 | 3.4 | 6.0 |
2AP-2 | 2.9 | 6.5 |
2AP-3 | 2.9 | 7.0 |
2AP-4 | 2.4 | 5.4 |
2AP-T | 1 | 1 |
2AP-(-)b | 12.6 | 15.5 |
2APc | 6.6 | 7.1 |
aThe ratio of fluorescence intensities at 370 nm for the 2AP-Y duplexes (F) and the 2AP-T duplex(F0): Buffer cond. c(duplex) = 2 µM, in 10 mM NaH2PO4, pH 7.0.
bDuplex with 2AP residue in a single nucleotide bulge.
c2AP containing single strand.