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. 2023 Jun 2;19(6):e1010781. doi: 10.1371/journal.pgen.1010781

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© 2023 Wurmser et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 4 — (A) Immunostaining on head coronal section of E16.5 Ctrl (n = 3) and Wnt1-Cre; Six1^Lox/Lox Six2^-/- (n = 3) fetuses showing impairments in EOM muscle formation by MF20 (green), Dapi (Blue); E: Eye, T: Tongue, M: Masseter, Sb = 200μm. (B) Immunostainings on transverse trunk sections for E18.5 Ctrl (s1 Hz), s1KO (n = 3), s2KO (n = 2) and s1s2dKO (n = 3) fetuses for sarcomeric myosins marked by MF20 (green) and the smooth muscle marked by Desmin (red); oe: esophagus, Sb = 30μm. (C) Left panel: Immunostainings on transverse trunk sections for E14.5 Ctrl and qKO (n = 3) fetuses showing sarcomeric myosins marked by MF20 (green), and Dapi (blue); NM: Neck Muscles, oe: esophagus, Sb = 300μm. Right panel: Immunostainings on transverse trunk sections for E18.5 Ctrl and qKO (n = 3) fetuses zoomed at the neck level showing sarcomeric myosins marked by MF20 (red), Pax7 (green) and Dapi (blue); NM: Neck Muscles, oe: esophagus, Sb = 150μm.