Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Antibody | Iba1 (Rabbit polyclonal) | Wako Chemicals | 019–19,741 | 1:400 |
| Antibody | Ki-67 (Rabbit monoclonal) | Abcam | ab16667 | 1:400 |
| Antibody | Anti-Rabbit IgG Alexa Fluor 594 (Goat-polyclonal) | Invitrogen | A11012 | 1:800 |
| Antibody | Anti-Rabbit IgG Alexa Fluor 647 (Chicken-polyclonal) | Invitrogen | A21443 | 1:800 |
| Antibody | CD45-BV421 (Rat-IgG2b monoclonal) | eBiosciences | 563890 | 1:350 |
| Antibody | CD11b-FITC (Rat-IgG2b monoclonal) | eBiosciences | 553310 | 1:250 |
| Antibody | CX3CR1-PE (Rat-IgG2a-K monoclonal) | Biolegend | 14906 | 1:250 |
| Antibody | Ly6G-APC (Rat-IgG2b monoclonal) | eBiosciences | 17-9668-82 | 1:250 |
| Antibody | Ly6C-PERCP (Rat-IgG2b monoclonal) |
eBiosciences | 45-5932-82 | 1:250 |
| Chemical Compound | PBS, pH 7.4 (1x) | Sigma-Aldrich | 10010023 | |
| Chemical Compound | Xylazine | Akorn | NDC59399-110-20 | |
| Chemical Compound | Ketamine | Par Pharmaceutical | NDC42023-115-10 | |
| Chemical Compound | Isoflurane | Cristália | 667940172 | |
| Chemical Compound | TRIzol | Thermo Fischer Scientific | 15596026 | |
| Chemical Compound | High-Capacity cDNA | Thermo Fischer Scientific | 4368814 | |
| Chemical Compound | SYBR Green Master Mix | Thermo Fischer Scientific | ||
| Chemical Compound | RPMI 1640 Medium | Gibco | 11875093 | |
| Chemical Compound | Ara-C | Sigma-Aldrich | 69-74-9 | |
| Chemical Compound | aCSF | Tocris | 3525 | |
| Chemical Compound | Collagenase type 2 | Worthington Biochemical Corporation | 9001-12-1 | |
| Chemical Compound | Fixable Viability Dye eF780 | Thermo Fischer Scientific | 65-0865-14 | |
| Chemical Compound | Triton X-100 | Sigma-Aldrich | T8787 | |
| Chemical Compound | Bovine Seruim Albumin (BSA) | Sigma-Aldrich | A7906-100G | |
| Chemical Compound | Sucrose | Fisher Scientific | S5-500 | |
| Chemical Compound | Tissue Tek | Electron Microscopy Sciences | 62550–01 | |
| Chemical Compound | Veet Hair Remover | Reckitt Benckiser | ||
| Software, algorithm | StepOne Real-Time PCR System | Thermo Fischer Scientific | 4376357 | |
| Software, algorithm | FACSAria III Cell Sorter | BD Biosciences | ||
| Software, algorithm | FACSVerse | BD Biosciences | ||
| Software | Prism | GraphPad | Version 8 | |
| Software | FlowJo | FlowJo | V10.8.1 | |
| Genetic Reagent (Mus musculus C57BL/6) | Cx3cr1 GFP | Jackson Laboratories | Strain# 005582 |
PMID:17944871 |
| Genetic Reagent (Mus musculus C57BL/6) | Ccr2 RFP | Jackson Laboratories | Strain# 017586 |
PMID:21060874 |
| Genetic Reagent (Mus musculus C57BL/6) | eGFP C57BL/6-(Tg[CAG-EGFP]) | Jackson Laboratories | Strain# 006567 |
PMID:9175875 |
| Genetic Reagent (Mus musculus C57BL/6) | Ms4a3Cre | Liu et al., 2019. | N/A | PMID:31491389 |
| Genetic Reagent (Mus musculus C57BL/6) | Rosa26tdTomato | Jackson Laboratories | Strain# 007914 |
PMID:20023653 |
| Sequence-based reagent | Gapdh_F | Sigma-Aldrich (This paper) | PCR primers | GGGTGTGAACCACGAGAAAT |
| Sequence-based reagent | Gapdh_R | Sigma-Aldrich (This paper) | PCR primers | CCTTCCACAATGCCAAAGTT |
| Sequence-based reagent | Aif1_F | Sigma-Aldrich (This paper) | PCR primers | GCTTCAAGTTTGGACGGCAG |
| Sequence-based reagent | Aif1_R | Sigma-Aldrich (This paper) | PCR primers | TGAGGAGCCATGAGCCAAAG |
| Sequence-based reagent | Cx3cr1_F | Sigma-Aldrich (This paper) | PCR primers | GCCTCTGGTGGAGTCTGCGTG |
| Sequence-based reagent | Cx3cr1_R | Sigma-Aldrich (This paper) | PCR primers | CGCCCAAATAACAGGCCTCAGCA |
| Sequence-based reagent | Cx3cl1_F | Sigma-Aldrich (This paper) | PCR primers | CGCGTTCTTCCATTTGTGTA |
| Sequence-based reagent | Cx3cl1_R | Sigma-Aldrich (This paper) | PCR primers | CTGTGTCGTCTCCAGGACAA |
| Sequence-based reagent | Il6_F | Sigma-Aldrich (This paper) | PCR primers | TTCCTACCCCAATTTCCAAT |
| Sequence-based reagent | Il6_R | Sigma-Aldrich (This paper) | PCR primers | CCTTCTGTGACTCCAGCTTATC |
| Sequence-based reagent | Tnf_F | Sigma-Aldrich (This paper) | PCR primers | GCCACAAGCAGGAATGAGAAG |
| Sequence-based reagent | Tnf_R | Sigma-Aldrich (This paper) | PCR primers | AGCAAGCAGCCAACCAGG |
| Sequence-based reagent | Kcnj1_F | Sigma-Aldrich (This paper) | PCR primers | GGGCTATCAGAGGCTGTGTC |
| Sequence-based reagent | Kcnj1_R | Sigma-Aldrich (This paper) | PCR primers | GTGACAGGCAAACTGCTTCA |
| Sequence-based reagent | Il1b_F | Sigma-Aldrich (This paper) | PCR primers | TGACAGTGATGATGAGAATGACCTGTTC |
| Sequence-based reagent | Il1b_R | Sigma-Aldrich (This paper) | PCR primers | TTGGAAGCAGCCCTTCATCT |
| Sequence-based reagent | Cfs1r_F | Sigma-Aldrich (This paper) | PCR primers | ACACGCACGGCCACCATGAA |
| Sequence-based reagent | Cfs1r_R | Sigma-Aldrich (This paper) | PCR primers | GCATGGACCGTGAGGATGAGGC |