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. 2023 Jun 15;133(12):e162088. doi: 10.1172/JCI162088

Figure 4. Neutrophils and NK cells contribute to IL-36–mediated antitumor responses in Rag-KO mice.

Figure 4

(A) IL-36R (Il1rl2) expression in various immune cells isolated from spleen (NK, DCs) or bone marrow (BMDMs, monocytes, neutrophils) of WT C57BL/6 mice, with the highest IL-36R content found in neutrophils. Normalized read counts from bulk RNA-Seq were used. (B) IL-36γ (Il1f9) expression in various immune cells isolated from spleen (NK, DCs) or bone marrow (BMDM, monocytes, neutrophils) of WT C57BL/6 mice either untreated or treated with 500 ng/mL rmIL-36γ for 6 hours. The y axis is split to show minimal expression in certain cell types. Normalized read counts from bulk RNA-Seq were used. (C) Tumor growth of control or IL-36γ–expressing MC38 syngeneic tumors in Rag-KO mice administered with various cell-depleting mAbs. Anti-NK1.1. mAbs were used to deplete NK cells, Ly6G mAbs were used to deplete neutrophils, and Gr-1 mAbs were used to deplete both neutrophils and monocytes. Tumor growth inhibition (TGI) and individual tumor growth curves are shown (n = 10/group). Data are shown as mean ± SEM. (B) Unpaired 2-tailed Mann-Whitney t test. (C) Two-way ANOVA followed by Tukey’s multiple comparison test. *P < 0.05, ***P < 0.001. neut, neutrophils; mono, monocytes. Data are representative of 1 (A and B) and 2 (C) independent experiments.