Figure 3. Loss or inhibition of DPP4 reduces cell-survival proteins BCL2L1 and BCL2L2.

(A) RT-qPCR analysis of RNA from hVSMCs transfected with siCtrl or siDPP4 and treated with DMSO, CoCl₂, or Doxo for 7 days. Graphs indicate the levels of DPP4, p21, LMNB1, BCL2L1, and BLC2L2 mRNAs in each group, normalized to ACTB mRNA levels. (B) RT-qPCR analysis of RNA from hVSMCs transfected with siCtrl or siDPP4 and treated with DMSO, CoCl₂, or Doxo for 7 days. The levels of DPP4, p21, LMNB1, BCL2L1, and BLC2L2 mRNAs were normalized to ACTB mRNA levels. (C) Western blot analysis of protein levels of p21, LMNB1, BCL2L1, BCL2L2, and loading control ACTB in hVSMCs processed as described in A. (D) Western blot analysis of the levels of p21, LMNB1, BCL2L1, BCL2L2, and loading control ACTB in hVSMCs treated as described in B. Data are represented as mean ± SD from n = 3 or indicated biological replicates. Significance was established using Student’s t test with Bonferroni’s correction. *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001; ****P ≤ 0.0001.