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. 2023 Jun 15;133(12):e165933. doi: 10.1172/JCI165933

Figure 6. DPP4 downstream targets enable senescent cell viability.

Figure 6

(A) Bioplex analysis of complement and coagulation proteins in human serum samples from people designated with low and high FRS (n = 50 per group). (B) Normalized protein intensity represented in a heatmap comparison of normal and pathological (atherosclerotic) aortic tissue from >62-year-old donors. (C) Percentage of cell viability graphed for each siRNA transfected into hVSMCs treated with Doxo (n = 2). The dashed line indicates 100% viability, and the red arrows point to siRNAs that resulted in approximately 50% reduction or increase in cell viability. (D and E) hVSMCs were treated with the indicated conditions for 7 days and micrographs were obtained (D) and caspase-3 and -7 activity levels were calculated (E). Original magnification, ×20. Data are represented as mean ± SD from n = 3 or indicated biological replicates. Significance was established using Student’s t test with Bonferroni’s correction. *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001.