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. 2023 Jun 14;14:3514. doi: 10.1038/s41467-023-39178-x

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© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2023

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Fig. 4 — ELISA analysis of library-selected cyclonal antibodies derived from genetic selection with Gcn4-PP as target antigen. Binding activity and specificity of clones were evaluated by ELISA using purified GST-Gcn4-PP, GST-HAG, GST-c-Myc, or GST-c-Jun as immobilized antigen. Data were obtained for the starting GFA cyclonal antibody (K_D = 2.7 nM) and for the CDR-H3 variants that were isolated by genetic selection, namely GLF, GLH, GLM, GIN, SLF, GTK, and GLLD. It should be noted that GLF, GLH, and GLM were the most abundantly selected clones from the three-residue library. Absorbance was measured at 450 nm and values for each CDR-H3 variant were normalized to the maximum value obtained for GLF. Data are the average of three biological replicates ± SD. Source data are provided as a Source Data file.