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. 2023 Jun 14;13:9657. doi: 10.1038/s41598-023-33915-4

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Correlation between expression of gene markers of macrophage, M2 macrophage and survival of BLCA patients based on data from Kaplan–Meier plotter. (B) Representative IHC staining of CD44, CD68, and CD163 protein in BLCA tumor sample. Upper: representative patient with high CD44 expression. Lower: representative patient with low CD44 expression. (C) Correlation analysis between the percentage of CD44 and the number of macrophages and M2 macrophages per patient. Pearson’s correlation coefficient (r) and significance level (P-value) are shown as correlations.