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. 2023 Jun 14;13:9630. doi: 10.1038/s41598-023-35952-5

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of extracts and compounds on proliferation of stimulated primary human lymphocytes. Primary human lymphocytes were stained with CFSE and stimulated with anti-CD3 and anti-CD28 mAbs. Stimulated cells were incubated for 72 h in the presence of medium (unstim., stim.), cyclosporine A (CsA; 4.16 μM), extracts or compounds. Cell division was analyzed by flow cytometry. Results were normalized to positive control for proliferation inhibition CsA and depicted as mean ± standard deviation. n = 3; *p < 0.05; **p < 0.01.