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Polysome/mRNP distribution of a TOP (rp-L4) mRNA and a non-TOP (S16CM3-GH) mRNA in growing and resting cells, in the presence or absence of overexpressed La. HEK293 cells were co-transfected with plamsids pXrp-L4 and pS16CM3-GH, with or without pUβG-Lawt. After transfection, cells were incubated under growing or resting conditions (see text), then lysed and analyzed as in the experiment described in Figure 3.
