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. 2023 Jun 1;5:1196697. doi: 10.3389/fgeed.2023.1196697

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Copyright © 2023 Granadillo Rodríguez, Wong and Chelico.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Early γH2AX foci in MCF10A- and MCF7-derived stable cell lines. The A3-Flag expression was either uninduced or induced with dox for 24 h in (A) MCF10A and (B) MCF7-derived stable cell lines before staining with antibodies. (C) For A3H Hap I, the procedure was repeated in MCF7 cells after dox induction for 72 h. The percentage of cells with γH2AX foci was determined. Two independent experiments were conducted, and the results from each field of view examined for each experiment are indicated with different symbols. Supplementary Figures S4, S5 show representative images from one experiment.