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. 2023 May 11;299(6):104805. doi: 10.1016/j.jbc.2023.104805

Figure 1.

Figure 1

Single cell RNA-seq reveals the heterogeneity of early mesenchymal cells in the limb bud.A–E, Single cell RNA-seq analysis of Prrx1-cre-marked limb bud mesenchymal cells at E11.5. A, Prrx1-cre; R26RtdTomato femur stained for SOX9. Scale bar: 200 μm. n = 3 mice. B and C, FACS (B) and scRNA-seq (C) strategy for Prrx1cre-tdTomato+ cells (red box). Shown are cells isolated from Prrx1-cre; R26RtdTomato limb buds (left) or R26RtdTomato control limb buds (right). D, UMAP-based visualization of major classes of Prrx1cre-tdTomato+ cells (Cluster 0–8). E, feature plots. Blue: high expression. Arrowheads: clusters in which a given gene is identified as a cell type-specific marker. Cluster 2,7: Sox9+, Cluster 3,5,6: Hes1+. Dotted contour: Sox9+ cells. n = 4804 cells. Pooled from n = 5 mice. F, RNAscope in situ hybridization analysis of E11.5 limb bud for Hes1 and Sox9 mRNA. Scale bar: 200 μm. n = 3 mice.