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. 2023 Jun 14;6(9):e202301920. doi: 10.26508/lsa.202301920

Figure S2. Drosophila Mtm rescues the cell size defect of dPIP4K29 independent of PI5P levels.

Figure S2.

(A) Graph representing average cell size measurement as mean ± S.E.M. of salivary glands from wandering third instar larvae of AB1/+ (n = 8) and AB1>MtmWT::GFP (n = 8). Sample size is also represented by points on individual bars. Unpaired t test with Welch correction showed P-value = 0.392. (B) (i) Protein levels between lysates made from Drosophila S2R+ cells. Lanes from left: untransfected control, mCherry vector and mCherry::Mtm observed on a Western blot probed by mCherry antibody. mCherry::Mtm migrates ∼100 kD. Tubulin was used as the loading control. (ii) In vitro phosphatase assay on synthetic PI(3,5)P2. Graph representing the formation of 18O-PIP2 formed from starting PI(3,5)P2 as substrate represented as mean ± S.E.M. on addition of either control (mCherry vector-transfected lysates) or mCherry::Mtm lysates. Lysate samples n = 3, where each sample was made from five third instar wandering larvae. Unpaired t test with Welch correction showed P-value = 0.696.