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. 2023 Jun 14;6(9):e202301920. doi: 10.26508/lsa.202301920

Figure S5. PI3P regulates cell size in salivary glands.

Figure S5.

(A) Immunoblot from the salivary glands of wandering third instar larvae probed using mCherry antibody showing expression of LTS::mCherry in AB1>LTS::mCherry (Control) and AB1>LTS::mCherry; dPIP4K29. Actin was used as the loading control. dPIP4K protein was checked in the samples to ascertain the mutant background. (B) Graph representing average cell size measurement (in μm3) as mean ± S.E.M. of salivary glands from wandering third instar larvae of AB1/+ (n = 7), AB1> MtmD402A::GFP (n = 7). Sample size is represented on individual bars. Unpaired t test with Welch correction showed P-value = 0.009. (C) Immunoblot from the salivary glands of wandering third instar larvae probed using mCherry antibody showing the expression of Atg8a-mCherry in AB1>Atg8a::mCherry (control) and AB1>Atg8a::mCherry; Mtm RNAi. Atg8a::mCherry migrates ∼42 kD. Tubulin was used as the loading control.