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. 2023 May 26;73:101744. doi: 10.1016/j.molmet.2023.101744

Figure 6.

Figure 6

Minar2 regulates mTORC1 activation and inhibits phosphorylation of raptor. (A) Immunofluorescence staining showing hyperphosphorylation (Ser2448) of mTOR in the adipocytes harvested from Minar2 KO mice or control WT mice. (B) Western blotting analysis showing expression of Minar2 in HEK-293 cells inhibits phosphorylation of mTOR on Ser2448. Cells were starved for 16 h, then stimulated with insulin (100 ng/mL, 30min), cells were lysed and whole cell lysates were subjected to western blot analysis. (C) In vitro mTORC1 kinase assay using GST-4EBP1 as a substrate. (D) Western blot analysis showing expression of Minar2 in HEK-293 cells inhibits phosphorylation of raptor on Ser863 and Ser877. (E) Summary and proposed model for interaction of Minar2 with Raptor. In normal physiological conditions, Minar2 interacts with Raptor and limits its interaction with mTORC1 complex, which leads to temporal regulation of mTORC1 activity. Inactivation of Minar2 disrupts the temporal regulation of mTORC1, leading to hyperactivation of mTORC1 signaling.