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. 2023 Apr 24;42(12):e112675. doi: 10.15252/embj.2022112675

Figure EV3. The enrichment of GDH1 for acetylation identification.

Figure EV3

  • A
    The successful knockin of FLAG into C terminal of GDH1 in HCT116 or SW480 cells was tested by western blot.
  • B
    The overall αKG level in HCT116 or SW480 cells with or without FLAG tagging endogenous GDH1 C terminal under normoxia and hypoxia (n = 3).
  • C, D
    MS/MS spectra identified K503 and K527 acetylation of GDH1 purified from HCT116 cells.

Data information: Data are mean ± SD from the biological replicates (B). Statistics: unpaired two‐tailed Student's t‐test (B).

Source data are available online for this figure.