Figure 7. AcK503/527‐GDH1 indicates a poor prognosis, mainly dependent on HIF1α stabilization.

• A, B
  Cell viability and percentage of apoptosis in the indicated cell lines under hypoxia were analyzed by trypan blue (A) and PI/Annexin V double staining (B), respectively (n = 3).
• C, D
  The xenograft tumor formation efficiency of the indicated cell lines was assessed, and tumor weight measurements were performed (n = 3).
• E
  Representative images of CRC samples are shown after antibody staining of AcK503‐GDH1 and AcK527‐GDH1.
• F
  The correlation between AcK503‐GDH1 and AcK527‐GDH1 expression in CRC samples. The tissue sections were quantitatively scored according to the percentage of positive cells and the staining intensity (H‐score) (n = 51).
• G
  Representative images of the same CRC samples are shown after staining with antibodies against AcK503/527‐GDH1 and HIF1α.
• H
  Correlation between AcK503/527‐GDH1 and HIF1α expression in CRC samples (n = 21).
• I
  Detection of AcK503/527‐GDH1 expression in CRC grades I + II and III + IV (grade I + II, n = 21; grade III + IV, n = 12).
• J
  Detection of AcK503/527‐GDH1 expression in patients with (+) or without (−) recurrence (−, n = 17; +, n = 17).
• K
  Patient survival corresponding to different levels of AcK503/527‐GDH1 expression (High, n = 38; Low, n = 32).
• L
  PDX growth related to high or low levels of AcK503/527‐GDH1 expression.
• M
  Schematic model described in the discussion section.

Data information: Data are mean ± SD from the biological replicates (A, B, D, I, J, L). Statistics: two‐way ANOVA with Tukey's HSD post hoc test (A, L); one‐way ANOVA with Tukey's HSD post hoc test (B, D); unpaired two‐tailed Student's t‐test (F, H); Mann–Whitney U‐test (I, J); log‐rank test (K).

Source data are available online for this figure.