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Gel mobility shift assays were performed with nuclear extracts from HT-29 cells and a 25-bp doubled-stranded Sp1 oligonucleotide probe in the absence (lane 1), or the presence of unlabeled wild-type (lane 2), or mutated Sp1 oligonucleotide (lane 3). The DNA–protein complex (C) was supershifted by the addition of GKLF antiserum in the reaction (SS, lane 4). Unlabeled competitors were added at 100-fold molar excess.
