Fig. 4.

Postnatal age-dependent changes in the number of different types of small intestinal epithelial cells and proliferation markers in mice. (A) Representative fluorescent images for immunostained frozen sections of Lyz1-positive paneth cells, Ki67-positive proliferative cells, ChgA-positive enteroendocrine cells (red), and DAPI stained nuclei (blue), obtained from the postnatal mice small intestine (ileum). (20× magnification, scale bar = 50 μm). Quantification of paneth cells (B), proliferative cells (C) in the intestinal crypts, and enteroendocrine cells (D) in the villus of neonate mice. (Data are represented as means ± SD from ≥8 crypts or villus from 3 animals per age group. ∗∗p ≤ 0.01, ∗∗∗p ≤ 0.001 and ∗∗∗∗p ≤ 0.0001; one-way ANOVA with Dunnett's multiple comparisons test and compared with 1 day).