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Luciferase-based β-arrestin 2 recruitment assay. After agonist binding, SmBiT-β-arrestin 2 migrates to the adenosine A1 receptor (A1AR) that is coupled to LgBiT. Binding of LgBiT and SmBiT results in a functional enzyme (NanoLuc) that generates light in the presence of the substrate coelenterazine h.
