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. 2000 Aug 1;28(15):2873–2881. doi: 10.1093/nar/28.15.2873

Figure 2.

Figure 2

Plasmid shuffling test of DNA2 mutants with its own promoter or the GAL1 promoter. (A) Strain YKH12 (YPH501 Δdna2::HIS pRS316-DNA2) was transformed with pRS314 vector only (vector), pRS314-DNA2 (wt) and pRS314-dna2-X (dna2-X) (the capital X denotes the number of the mutant allele constructed). Expression of the mutant alleles was carried out under the control of the native promoter of DNA2. Transformants were grown to saturation and serial dilutions placed on complete synthetic medium (containing glucose) lacking tryptophan in the absence (–FOA) or presence (+FOA) of 5-FOA. (B) Strain YKH12 was transformed with pRS314GU vector only (vector), pRS314GU-DNA2 (wt) and pRS314GU-dna2-X (dna2-X). Expression of the mutant alleles was carried out under control of the GAL1 promoter. Transformants were grown and plated in complete synthetic medium lacking tryptophan in the absence (–FOA) or presence (+FOA) of 5-FOA. Glucose was used as carbon source (Glucose) to repress expression of the DNA2 mutant alleles. (C) The same transformants used for (B) were plated in complete synthetic medium that contained galactose as carbon source (Galactose). They were grown in the absence (–FOA) or presence (+FOA) of 5-FOA. All plates were grown for 4 days at 30°C.