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Plk1 regulates nestin phosphorylation at Thr-315 in smooth muscle. A Protein expression of Ctrl and Plk1 KD HASM cells were evaluated by immunoblot analysis. B Ctrl and Plk1 KD HASM cells were treated with 10–4 M ACh for 5 min, or left untreated. Nestin phosphorylation was determined by immunoblot analysis. Plk1 KD inhibits ACh-induced nestin phosphorylation at Thr-315. Data are means ± SE (n = 4–5 batches of culture from 3 donors). * p < 0.05
