Table 2.
Test results of drug-dependent antibodies in blood samples collected 2 days before and 3 days after stopping the administration of piperacillin-tazobactam
| NO. | Reactive materials | Results of the antiglobulin testa | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| P-P (μl) |
AE (μl) |
AB-P (μl) |
3 mg/ml PRC (μl) |
1 mg/ml TBT (μl) |
RBCs (μl) |
PRC-RBCs (μl) |
TBT-RBCs (μl) |
2 days before stopping | 3 days after stopping | |
| 1 | 100 | / | / | 100 | / | 50 | / | / | 4 + | 4 + |
| 2 | 100 | / | / | / | 100 | 50 | / | / | - | - |
| 3 | / | 100 | / | 100 | / | 50 | / | / | - | - |
| 4 | / | 100 | / | / | 100 | 50 | / | / | - | - |
| 5 | 100 | / | / | / | / | 50 | / | / | 3 + b | - |
| 6 | 100 | / | / | / | / | / | 50 | / | 3 + b | - |
| 7 | 100 | / | / | / | / | / | / | 50 | 3 + b | - |
| 8 | / | 100 | / | / | / | 50 | / | / | - | - |
| 9 | / | 100 | / | / | / | / | 50 | / | - | - |
| 10 | / | 100 | / | / | / | / | / | 50 | - | - |
| 11 | / | / | 100 | 100 | / | 50 | / | / | - | - |
| 12 | / | / | 100 | / | / | 50 | / | / | - | - |
| 13 | / | / | 100 | / | / | / | 50 | / | - | - |
| 14 | / | / | 100 | / | / | / | / | 50 | - | - |
P-P Patient’s plasma, AE Patient’s acid eluent, AB-P AB-type plasma with negative antibody screening test result, PRC Piperacillin, TBT Tazobactam, RBCs Uncoated O-type red blood cells, PRC-RBCs Piperacillin-coated red blood cells, TBT-RBCs Tazobactam-coated red blood cells, 2 days before stopping 2 days before stopping the administration of piperacillin-tazobactam, 3 days after stopping 3 days after stopping the administration of piperacillin-tazobactam
aThe same methods were used to detect drug-dependent antibodies in blood samples collected on day 2 before and on day 3 after the patient’s stopping of the administration of piperacillin-tazobactam, and the direct antiglobulin tests were used to confirm the presence of drug-dependent antibodies
bDue to the presence of piperacillin in the patient’s plasma before stopping the administration of piperacillin-tazobactam, the plasma reacted with both drug-coated red blood cells and uncoated red blood cells; + : strong; -: negative
Remarks: All the 14 combinations of reactive materials were incubated at 37ºC for 1 h and observed by centrifugation (no agglutination and hemolysis were found), and then performed the antiglobulin tests to detect drug-dependent antibodies