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. 2023 Jun 15;18:31. doi: 10.1186/s13062-023-00381-x

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — LncRNA and mRNA expression in three generations mouse testis samples by RNA-seq analysis. (a) Study scheme. Gene changes in sperm of three generations of mice treated with NH₃/H₂S. (b) The number of known up-regulated lncRNAs in the sperm of three generations of mice after NH₃/H₂S treatment. (c) The number of known increased mRNAs in the sperm of three generations of mice after NH₃/H₂S treatment. (d) GO enrichment of target genes that known up-regulated lncRNAs in F0 generation. (e) The expression of lncRNA5251 in the sperm of three generations of mice