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. 2023 Jun 15;18:31. doi: 10.1186/s13062-023-00381-x

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Spermatogenesis was upset in F1 mice after overexpression lncRNA5251. (a) The protein staining of DDX4, SYCP3, TP1 and PGK2 in F1 mice testis after shRNA treatment by IHF. (b) The quantitative data for IHF in (a). (c) GO enrichment analysis of up-regulated genes after inhibiting lncRNA5251. (d) GO enrichment analysis of down-regulated genes after overexpressing lncRNA5251. (e) The protein levels of CADM2, GDNF and ODF1 detected by WB in F1 mouse testes. (f) The quantitative data for WB in (e). Data present as Average ± SEM. a, b indicate a significant difference among different treatments (p < 0.05)