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Time course (Rn versus time) for cleavage of S by E in the presence of various concentrations of Mg2+ ion: 50 mM (closed squares); 25 mM (open circles); 12.5 mM (closed triangles); 5 mM (crosses); 2.5 mM (open squares); 1.25 mM (closed circles); 0 mM (open triangles); c(E) = 100 nM, c(S) = 3 µM.
