FIG 3.

Cryo-EM data collection and processing. (A) Grid screening revealed the desired protein particles that resembled either a rod or eclipse shape. (B) Strategy of image analysis. (C) The acceptable 2D classes revealed rod- and eclipse-like structures. (D) A 4.6-Å structure was determined and further transferred to RELION v3.1 for Bayesian polishing, resulting in a final structure with a global 4.52-Å resolution. (E) The local resolution of the various regions was highlighted in a red-white-blue scale. The lowest resolution of 4 Å is represented by blue, and the highest resolution of 7 Å is represented by red.