TABLE 1.
Cryo-EM data acquisition and image processing of M. tuberculosis cyt-bcc:aa3
| Data acquisition and image processing | M. tuberculosis cyt-bcc:aa3 |
|---|---|
| Data collection | |
| Electron microscope | Titan Krios |
| Camera | Gatan K2 |
| Mode | Super-resolution counting |
| Voltage (kV) | 300 |
| Nominal magnification | 130,000 × |
| Calibrated physical pixel size (Å) | 1.06 |
| Exposure time (s) | 9 |
| Total exposure (e–/Å2) | 40 |
| No. of frames | 40 |
| Defocus range (μM) | 1.0–2.0 |
| Image processing | |
| Motion correction software | MotionCor2 |
| CTF estimation software | CTFFind4 |
| Particle selection software | cryoSPARC v3.3.1 |
| 2D classifications | cryoSPARC v3.3.1 |
| 3D classification and refinement software | RELION v3.1 |
| Particle motion correction software | RELION v3.1 |