FIG 3.

l-arginine biosynthesis-dependent peroxide killing is exacerbated in the presence of oxygen. Survival of wild-type (WT) and ΔargCBH mutant Salmonella grown overnight in EG minimal media supplemented with 20 μg/mL l-arginine after 30 min of treatment with 400 μM H₂O₂ in EG supplemented with 80 μg/mL l-arginine. (a) Salmonella in aerobic experiments were grown overnight and treated with H₂O₂ in the presence of oxygen. (b) Salmonella in aerobic to anaerobic shift experiments were grown overnight in the presence of oxygen and then treated with H₂O₂ under anaerobic conditions. (c) Salmonella in anaerobic experiments were grown overnight and treated with H₂O₂ under anaerobic conditions. Data are shown as means ± SEM (n = 6) and was analyzed by two-way ANOVA with Tukey’s correction where *, P < 0.05; ****, P < 0.0001.