TABLE 2.
MIC values of antibiotics for E. cloacae clinical isolates, E. cloacae ATCC 13047, and E. coli CFT073 (and their derivative strains)
| Strain | MIC (mg/L [fold change])a |
|||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AMX | TEM | TIC | PIP | FOX | CTX | FEP | ATM | ERT | GEN | CIP | TGC | CST | CHL | |
| E. asburiae | ||||||||||||||
| Temo_S | >256 | 4 | 2 | 4 | >256 | 0.5 | 0.03 | 0.03 | 0.25 | 0.12 | <0.01 | 0.5 | 32 | 4 |
| Temo_R | >256 | 32 (8) | 64 (32) | 4 | >256 | 0.5 | 0.03 | 0.12 (4) | 0.25 | 0.12 | <0.01 | 0.5 | 64 | 4 |
| Temo_S/pBAD | >256 | 4 | 2 | 2 | >256 | 0.5 | 0.03 | 0.03 | 0.25 | 0.12 | <0.01 | 0.5 | 32 | 4 |
| Temo_S/pBADΩmdtABCD | >256 | 4 | 8 (4) | 2 | >256 | 0.5 | 0.03 | 0.03 | 0.25 | 0.12 | <0.01 | 0.5 | 16 | 4 |
| Temo_S/pBADΩacrD | >256 | 64 (16) | 64 (32) | 2 | >256 | 0.5 | 0.03 | 0.12 (4) | 0.25 | 0.12 | <0.01 | 0.5 | 32 | 4 |
| E. cloacae subsp. cloacae | ||||||||||||||
| ATCC 13047 | >256 | 8 | 4 | 8 | >256 | 2 | 0.03 | 0.5 | 0.25 | 0.25 | 0.03 | 0.5 | >256 | 4 |
| ATCC 13047ΔbaeS | >256 | 4 | 4 | 8 | >256 | 2 | 0.03 | 0.5 | 0.25 | 0.25 | 0.03 | 0.5 | 256 | 4 |
| ATCC 13047ΔmdtB | >256 | 8 | 4 | 8 | >256 | 2 | 0.03 | 0.5 | 0.25 | 0.25 | 0.03 | 0.5 | 256 | 4 |
| ATCC 13047ΔacrD | >256 | 8 | 4 | 8 | >256 | 2 | 0.03 | 0.25 | 0.25 | 0.25 | 0.03 | 0.5 | >256 | 4 |
| ATCC 13047/pBAD | >256 | 8 | 4 | 8 | >256 | 2 | 0.03 | 0.5 | 0.25 | 0.25 | 0.03 | 0.5 | >256 | 4 |
| ATCC 13047/pBADΩmdtABCD | >256 | 8 | 16 (4) | 8 | >256 | 2 | 0.03 | 0.5 | 0.5 | 0.25 | 0.03 | 0.5 | >256 | 4 |
| ATCC 13047/pBADΩacrD | >256 | 64 (8) | 128 (16) | 8 | >256 | 2 | 0.03 | 2 (4) | 0.25 | 0.25 | 0.03 | 0.5 | >256 | 4 |
| E. coli | ||||||||||||||
| CFT073 | 4 | 4 | 4 | 2 | 2 | 0.12 | 0.06 | 0.06 | 0.06 | 0.12 | <0.01 | 0.12 | 0.12 | 4 |
| CFT073_baeSThr175Pro | 4 | 64 (16) | 64 (16) | 2 | 2 | 0.12 | 0.06 | 1 (16) | 0.06 | 0.12 | <0.01 | 0.12 | 0.12 | 4 |
a
Numbers in bold are MICs that are significantly different (fold changes of ≥4 are indicated in parentheses). AMX, amoxicillin; ATM, aztreonam; CHL, chloramphenicol; CIP, ciprofloxacin; CST, colistin; CTX, cefotaxime; ERT, ertapenem; FEP, cefepime; FOX, cefoxitin; GEN, gentamicin; PIP, piperacillin; TEM, temocillin; TIC, ticarcillin; TGC, tigecycline.