FIG 1.

OmpR is required for hypermucoviscosity of K. pneumoniae. (A) OmpR deficiency reduced hypermucoviscosity in three K2 serotype strains (ATCC43816, TH12887, and TH13044) and one serotype K23 strain (TH12896). The ompR^comp is the complementation of the ΔompR mutant, and Δwzab capsule-null mutant is a negative control. (B) Effects of phosphorylation of OmpR on K. pneumoniae mucoviscosity. The ompR^D55A encodes the unphosphorylated form of OmpR, and ΔenvZ is a histidine kinase EnvZ deletion mutant. Data are presented as mean ± SD from six biological replicates. Two-tailed t test and one-way ANOVA with Dunnett’s multiple-comparison test were performed to determine the statistical significance of two group comparisons. ***, P < 0.001; ****, P < 0.0001; ns, no significance.