FIG 2.

Characterization of the SVA-specific MAbs 1M5 and 1M25. (A) Detection of the reactivity of MAbs 1M5 and 1M25 to SVA antigen by indirect ELISA. The MAbs with different concentrations were added to SVA antigen-coated ELISA plates and incubated at 37°C for 1 h. Then, HRP-conjugated rabbit anti-pig antibody was added, and the OD₄₅₀ values were measured using an automatic microplate reader. The results were obtained from three biological replicates (mean ± standard deviation). (B) Detection of the reactivity of MAbs 1M5 and 1M25 to SVA by IFA. BHK-21 cells were mock infected or infected with SVA and identified via IFA using 1M5, 1M25, anti-SVA-positive serum, and anti-SVA-negative serum. (C) Detection of the reactivity of MAbs 1M5 and 1M25 to SVA structural proteins by IFA. BHK-21 cells were transfected with plasmid containing the SVA VP1, VP2, VP3, or VP4 gene, respectively. The cells were then fixed and immunostained with 1M5, 1M25, and anti-Flag MAb followed by specific secondary antibody.