FIG 5.

Differences in MFI of strains JE2, MRSA 11/11, BMC1001, and COL grown with and without NaHCO₃ in the presence of 1/2 MIC of oxacillin for (A) transcriptional (txn) and (B) translational (tln) constructs. The constructs and conditions used are indicated in the legend to Fig. 4. Each bar shows the difference (increase/decrease) between the MFIs of the strain grown with and without NaHCO₃ (baseline levels), and the statistical significance between them was calculated using one-way ANOVA followed by Tukey’s multiple-comparison test. ns, not significant (P > 0.05); *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. Data are the results from four independent biological replicates for each strain/condition.