TABLE 5.
Conidial viability parameters after 96 h of exposure to unfermented C. subternata extracta
| Fusarium strain | % ATP production |
IC50 of ATP (mg/mL) | |||||||
|---|---|---|---|---|---|---|---|---|---|
| Control | Unfermented C. subternata extract |
AmpB (2.5 mg/mL) | |||||||
| 20 mg/mL | 10 mg/mL | 5 mg/mL | 2.5 mg/mL | 1.25 mg/mL | 0.62 mg/mL | ||||
| F. verticillioides | |||||||||
| MRC 826-E | 100 ± 0.67 | 76.7 ± 0.94*** | 84.2 ± 1.91*** | 85.3 ± 1.76*** | 158.9 ± 8.5*** | 164.6 ± 11.8*** | 196.6 ± 13.1*** | 50.1 ± 4.3*** | 32.95 ± 0.6** |
| MRC 826-J | 100 ± 1.11 | 30.6 ± 2.01*** | 34.0 ± 3.01*** | 53.2 ± 2.17*** | 56.6 ± 2.23*** | 70.9 ± 3.35*** | 86.4 ± 2.83*** | 23.3 ± 5.3*** | 4.83 ± 0.33** |
| MRC 8559 | 100 ± 1.35 | 23.9 ± 1.79*** | 25.4 ± 1.63*** | 59.5 ± 1.79*** | 78.1 ± 2.35*** | 88.5 ± 7.49*** | 110.4 ± 2.16*** | 2.08 ± 0.2*** | 6.23 ± 0.17** |
| F. subglutinans | |||||||||
| MRC 8554 | 100 ± 5.00 | 34.6 ± 2.24*** | 67.6 ± 2.93*** | 73.4 ± 0.84*** | 103.3 ± 3.8*** | 106.4 ± 1.95*** | 131.1 ± 7.21*** | 1.77 ± 0.3*** | 13.87 ± 0.39** |
| MRC 8553 | 100 ± 2.11 | 44.9 ± 2.14*** | 47.6 ± 0.84*** | 49.7 ± 1.72*** | 103.7 ± 1.8*** | 111.7 ± 7.00*** | 116.6 ± 2.47*** | 3.51 ± 0.1*** | 10.83 ± 0.47** |
| F. proliferatum | |||||||||
| MRC 8550 | 100 ± 3.19 | 87.1 ± 5.35*** | 87.7 ± 6.35*** | 99.9 ± 2.73*** | 116.0 ± 3.4*** | 109.6 ± 6.40*** | 143.9 ± 4.59*** | 44.1 ± 4.1*** | 45.88 ± 11.3** |
| MRC 8549 | 100 ± 3.34 | 24.9 ± 1.05*** | 56.1 ± 2.53*** | 82.9 ± 3.71*** | 96.4 ± 4.4*** | 114.8 ± 2.86*** | 138.9 ± 2.33*** | 45.2 ± 1.2*** | 11.33 ± 0.45** |
| F. graminearum MRC 4927 | 100 ± 2.84 | 47.8 ± 2.26*** | 63.4 ± 2.76*** | 72.4 ± 2.55*** | 111.3 ± 3.2*** | 116.6 ± 2.75*** | 127.6 ± 3.74*** | 0.21 ± 0.0*** | 17.17 ± 0.43** |
| F. globosum | |||||||||
| MRC 6647 | 100 ± 1.95 | 28.6 ± 1.54*** | 43.3 ± 2.36*** | 50.9 ± 2.02*** | 54.2 ± 2.4*** | 67.5 ± 3.17*** | 83.3 ± 2.08*** | 27.1 ± 4.2*** | 4.98 ± 0.23** |
| MRC 6122 | 100 ± 1.99 | 30.7 ± 1.28*** | 47.8 ± 1.34*** | 53.5 ± 1.69*** | 70.2 ± 1.17*** | 80.9 ± 2.41*** | 99.0 ± 1.61*** | 24.5 ± 2.7*** | 7.56 ± 0.32** |
a
Values represent means ± standard deviations of five replications from at least two experiments. % of ATP production, percentage of spore viability of cell energy production; IC50 of ATP, MIC at 50% ATP; *** and **, statistically significant at P < 0.0001 and P < 0.001, respectively.