Virulence potential of the HiRiC ST1971 strains. Virulence potential of six strains, including the HiRiC ST1971 strains b164, R530, and 19C16, ST381 types of strain Q410, and control strain PAO1, were evaluated in a zebrafish larva infection model (n = 100). The survival rates of zebrafish larvae infected by corresponding strains (400 CFU) are presented in various colors. Statistical analysis was performed by using a log-rank (Mantel-Cox) test and GraphPad Prism 8.0. ****, P < 0.0001; ***, P = 0.0002; **, P = 0.0058; *, P = 0.0428; ns, P = 0.6681.