Figure 2. Standard curve and LOD plots.

(A) Standard Curve plot with black circles representing the replicates of standards that were included in the linear regression calculations, the blue + indicated those reactions that were excluded from the linear regression calculations. The LOD of the assay is indicated by the solid line and the LOQ by the dotted line. (B) LOD plot indicating detection probability of each standard tested and coloured points represent the effective LOD for multiple replicate analysis as determined by a two parameter Weibell type 2 function.