Table 2. eDNA samples (water and plankton) collected from three sites in the lower Swan River (WA) in 2019 and tested using qPCR assay for the detection of Charybdis japonica. All samples were negative.

Date	Sample type	Sample code	Site	qPCR result	Latitude	Longitude
18/03/2019	eDNA water sample	A0158	Blackwall Reach	Negative	−32.0166	115.7851
18/03/2019	eDNA water sample	A0157	Blackwall Reach	Negative	−32.0180	115.7848
18/03/2019	eDNA water sample	A0159	Blackwall Reach	Negative	−32.0194	115.7842
18/03/2019	Plankton tow	A0160	Blackwall Reach	Negative	−32.0183	115.7851
18/03/2019	Plankton tow	A0161	Blackwall Reach	Negative	−32.0160	115.7844
19/03/2019	eDNA water sample	A0162	Mosman Bay	Negative	−32.0075	115.7725
19/03/2019	Plankton tow	A0165	Mosman Bay	Negative	−32.0075	115.7729
19/03/2019	eDNA water sample	A0163	Mosman Bay	Negative	−32.0085	115.7731
19/03/2019	Plankton tow	A0166	Mosman Bay	Negative	−32.0096	115.7730
19/03/2019	eDNA water sample	A0164	Mosman Bay	Negative	−32.0100	115.7745
27/03/2019	eDNA water sample	A0168	B52	Negative	−31.9787	115.8359
27/03/2019	Plankton tow	A0169	B52	Negative	−31.9778	115.8367
27/03/2019	eDNA water sample	A0171	B52	Negative	−31.9787	115.8353
27/03/2019	Plankton tow	A0170	B52	Negative	−31.9793	115.8359
27/03/2019	eDNA water sample	A0172	B52	Negative	−31.9790	115.8363