Table 3. Sequences of primers and TaqMan-MGB probe. Primer and probe name, sequence, attributed dye (probe), melting temperature (Tm, °C), GC content (%) and length (bp).
Name | Sequence 5′-3′ | Label | Tm C | %GC | Length (bp) |
---|---|---|---|---|---|
Cjap XL F | TTAATATACGGTCATTTGGTATGAGTATAGATC | _ | 59.5 | 30.3 | 33 |
Cjap XL R | AAGTTTCGGTCTGTTAATAATATAGTAATAGCT | _ | 59.4 | 27.3 | 33 |
Cjap X probe | TATTACTGCCATTCTTCTAC | FAM | 69.0 | 50 | 20 |